Mechanical Engineering of a Cryo STXM at CLS.
This analysis technique uses infrared radiation, and as the Infrared radiation is passed through the sample, where by some of it is absorbed and the rest of the infrared radiation is transmitted through. The spectrum that is created is a representation of the molecular absorption and transmission, which is unique to the sample.
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The analysis of spectra of chromosomes using the scanning transmission X-ray microscope (STXM) and its superposition of the pattern with the atomic force microscopy (AFM) and scanning electron microscopy (SEM) images proves that it is possible to precisely locate the gene loci and the DNA packaging inside the chromosomes.
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The analysis of the detailed energy map fitted with reference spectra of DNA and protein using STXM (Figure 4) reveals that the quinoa chromosome is primarily composed of DNA and protein, with some non-carbon components present inside and outside the chromosomes (X-ray image recorded at 280.0 eV). Proteins from plants and animals do not have differences in the spectral signatures due to the.
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STXM images and the XAS spectrum of CS and P22 on the Fe L-edge energy range are displayed in Fig. 3. In Fig. 3a, the STXM image includes red, blue, and green colors. The number indicates the XAS analysis points in the corresponding figure. The same color indicates that the regions have similar oxidation state.